Potential mechanisms for osteopathic manipulative treatment to alleviate migraine-like pain in female rats
Title
Potential mechanisms for osteopathic manipulative treatment to alleviate migraine-like pain in female rats
Date
2024
Publisher
Frontiers Media
Subject
osteopathic manipulative treatment
migraine-like headache
allodynia
trigeminal nucleus caudalis
trigeminal ganglia
calcitonin gene-related peptide
migraine-like headache
allodynia
trigeminal nucleus caudalis
trigeminal ganglia
calcitonin gene-related peptide
Language
English
Abstract
Introduction: Migraines are the leading cause of disability in the United States, and the use of non-pharmaceutical treatments like osteopathic manipulative treatment (OMT) has shown promise. Despite its potential, the lack of mechanistic understanding has hindered widespread adoption. This study aims to investigate the efficacy of OMT in treating acute migraines and unravel its underlyingmechanisms of action.
Methods: Female rats were subjected to a “two-hit” approach to induce migrainelikepain. This involved bilateral injections of Complete Freund’s Adjuvant (CFA) intothe trapezius muscle (1st hit) followed by exposure to Umbellulone, a human migraine trigger, on Day 6 post-CFA (2nd hit). Soft tissue and articulatory techniques were applied to the cervical region for acute abortive or repeated prophylactic treatment. Cutaneous allodynia and trigeminal system activation were assessed through behavioral tests and immunohistochemical staining.
Results: Following Umbellulone inhalation, CFA-primed rats exhibited periorbital and hind paw allodynia. Immediate application of OMT after Umbellulone inhalation as an abortive treatment partially alleviated cutaneous allodynia. With OMT applied thrice as a prophylactic measure, complete suppression of tactile hypersensitivity was observed. Prophylactic OMT also prevented the increase of c-fos signals in the trigeminal nucleus caudalis and the elevation of calcitonin gene-related peptide expression in trigeminal ganglia induced by CFA and Umbellulone exposure at 2 h post-inhalation.
Discussion: These findings provide mechanistic insights into OMT’s migraine-relief potential and underscore its viability as a non-pharmacological avenue for managing migraines.
Methods: Female rats were subjected to a “two-hit” approach to induce migrainelikepain. This involved bilateral injections of Complete Freund’s Adjuvant (CFA) intothe trapezius muscle (1st hit) followed by exposure to Umbellulone, a human migraine trigger, on Day 6 post-CFA (2nd hit). Soft tissue and articulatory techniques were applied to the cervical region for acute abortive or repeated prophylactic treatment. Cutaneous allodynia and trigeminal system activation were assessed through behavioral tests and immunohistochemical staining.
Results: Following Umbellulone inhalation, CFA-primed rats exhibited periorbital and hind paw allodynia. Immediate application of OMT after Umbellulone inhalation as an abortive treatment partially alleviated cutaneous allodynia. With OMT applied thrice as a prophylactic measure, complete suppression of tactile hypersensitivity was observed. Prophylactic OMT also prevented the increase of c-fos signals in the trigeminal nucleus caudalis and the elevation of calcitonin gene-related peptide expression in trigeminal ganglia induced by CFA and Umbellulone exposure at 2 h post-inhalation.
Discussion: These findings provide mechanistic insights into OMT’s migraine-relief potential and underscore its viability as a non-pharmacological avenue for managing migraines.
Source
Frontiers in Pain Research, February 2024, 1280589
Rights
© 2024 Byrd, Lund, Pan, Chung, Child, Fowler, Burns-Martin, Sanikommu, Henderson, Gregory, Fleming and Xie.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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Citation
Katherine Byrd, Makayla Lund, Yan Pan, Brandon H. Chung, Kaitlyn Child, Danny Fowler, Jared Burns-Martin, Mythili Sanikommu, Hallie Henderson, Caroline Gregory, Regina K. Fleming, Jennifer Yanhua Xie, Potential mechanisms for osteopathic manipulative treatment to alleviate migraine-like pain in female rats. Frontiers in Pain Research, February 2024, 1280589, New York Tech Institutional Repository, accessed April 27, 2024, https://repository.nyitlibrary.org/items/show/3797
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