Use of the Western blot technique to identify the immunogenic proteins of Borrelia burgdorferi for developing a Lyme disease vaccine
Title
Use of the Western blot technique to identify the immunogenic proteins of Borrelia burgdorferi for developing a Lyme disease vaccine
Date
2022
Publisher
Elsevier BV
Subject
Antibodies
Borrelia burgdorferi
Flagellin
Lyme disease
Vaccine
Western blot
Borrelia burgdorferi
Flagellin
Lyme disease
Vaccine
Western blot
Language
English
Abstract
Background
Lyme disease is a serious infectious disease having a restricted worldwide distribution for which there is no vaccine available for human use.
Objective
This study was designed to determine common reactive antigens involved in Borrelia burgdorferi (Bb) infection that are recognized in mammalian sera that may be useful for vaccine development.
Methods
Blood samples were collected from patients with documented Lyme disease, and from rabbits and mice experimentally infected with either tick-transmitted or culture-grown Borrelia burgdorferi . All samples were then processed for sera. For performing the Western blots, sonicated Bb organisms (whole cell lysates) and protein ladders were separated by protein gel electrophoresis. Immune reactivities of the electrophoresed proteins with the serum samples were then probed with anti-HRP IgG reagent.
Results
Rabbit, mouse and human sera consistently reacted with the 41 kDa band of Bb which corresponded to the flagellin protein – the major protein component of this organism’s periplasmic flagella, also known as axial filaments or fibrils. Various other Bb antigens of wide molecular weight ranges were also recognized by rabbit and human sera, and less frequently with mouse sera.
Conclusion
The strong immune response to the 41 kDa flagellin protein by the different mammalian species suggests the utility of a possible vaccine targeting this protein, although other proteins may also be appropriate, for preventing Lyme disease following a bite from an infected tick.
Lyme disease is a serious infectious disease having a restricted worldwide distribution for which there is no vaccine available for human use.
Objective
This study was designed to determine common reactive antigens involved in Borrelia burgdorferi (Bb) infection that are recognized in mammalian sera that may be useful for vaccine development.
Methods
Blood samples were collected from patients with documented Lyme disease, and from rabbits and mice experimentally infected with either tick-transmitted or culture-grown Borrelia burgdorferi . All samples were then processed for sera. For performing the Western blots, sonicated Bb organisms (whole cell lysates) and protein ladders were separated by protein gel electrophoresis. Immune reactivities of the electrophoresed proteins with the serum samples were then probed with anti-HRP IgG reagent.
Results
Rabbit, mouse and human sera consistently reacted with the 41 kDa band of Bb which corresponded to the flagellin protein – the major protein component of this organism’s periplasmic flagella, also known as axial filaments or fibrils. Various other Bb antigens of wide molecular weight ranges were also recognized by rabbit and human sera, and less frequently with mouse sera.
Conclusion
The strong immune response to the 41 kDa flagellin protein by the different mammalian species suggests the utility of a possible vaccine targeting this protein, although other proteins may also be appropriate, for preventing Lyme disease following a bite from an infected tick.
Source
Biomedicine & Pharmacotherapy, Volume 157, Article 114013, January 2023
Rights
Copyright © 2022 The Authors Published by Elsevier Masson SAS. This is an open-access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Format
PDF
Type
Text
Identifier
Bibliographic Citation
Loomba, K., Shi, D., Sherpa, T., Chen, J., Daniels, T. J., Pavia, C. S., & Zhang, D. (2023). Use of the Western blot technique to identify the immunogenic proteins of Borrelia burgdorferi for developing a Lyme disease vaccine. In Biomedicine & Pharmacotherapy (Vol. 157, p. 114013). Elsevier BV. https://doi.org/10.1016/j.biopha.2022.114013
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Collection
Citation
Loomba K., Shi D., Sherpa T., Chen J., Daniels T.J., Pavia C.S., Zhang D., Use of the Western blot technique to identify the immunogenic proteins of Borrelia burgdorferi for developing a Lyme disease vaccine. Biomedicine & Pharmacotherapy, Volume 157, Article 114013, January 2023, New York Tech Institutional Repository, accessed April 28, 2024, https://repository.nyitlibrary.org/items/show/3663
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