https://repository.nyitlibrary.org/files/original/a73a0e3ff109e83368f65ecf186956bb.pdf]]>
https://repository.nyitlibrary.org/files/original/afeec691054359693f9364830d76f7c4.pdf]]>
Calcium
Junctophilin-2
Ryanodine receptors
STORM
Thyroid hormone
Transverse-tubules]]>
Journal of Molecular and Cellular Cardiology, Volume 160, November 2021, pages 1-14]]>
Copyright © 2021 The Authors. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.]]>
https://repository.nyitlibrary.org/files/original/477666d47631cb0e9d1bb97dae489873.pdf]]>
https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
Gilani, N., Wang, K., Muncan, A., Peter, J., An, S., Bhatti, S., Pandya, K., Zhang, Y., Tang, Y.-D., Gerdes, A. M., Stout, R. F., & Ojamaa, K. (2021). Triiodothyronine maintains cardiac transverse-tubule structure and function. In Journal of Molecular and Cellular Cardiology (Vol. 160, pp. 1–14). Elsevier BV. https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
Calcium
Junctophilin-2
Ryanodine receptors
STORM
Thyroid hormone
Transverse-tubules]]>
Subclinical hypothyroidism and low T3 syndrome are commonly associated with an increased risk of cardiovascular disease (CVD) and mortality. We examined effects of T3 on T-tubule (TT) structures, Ca2+ mobilization and contractility, and clustering of dyadic proteins. Thyroid hormone (TH) deficiency was induced in adult female rats by propyl-thiouracil (PTU; 0.025%) treatment for 8 weeks. Rats were then randomized to continued PTU or triiodo-L-thyronine (T3; 10 μg/kg/d) treatment for 2 weeks (PTU + T3). After in vivo echocardiographic and hemodynamic recordings, cardiomyocytes (CM) were isolated to record Ca2+ transients and contractility. TT organization was assessed by confocal microscopy, and STORM images were captured to measure ryanodine receptor (RyR2) cluster number and size, and L-type Ca2+ channel (LTCC, Cav1.2) co-localization. Expressed genes including two integral TT proteins, junctophilin-2 (Jph-2) and bridging integrator-1 (BIN1), were analyzed in left ventricular (LV) tissues and cultured CM using qPCR and RNA sequencing. The T3 dosage used normalized serum T3, and reversed adverse effects of TH deficiency on in vivo measures of cardiac function. Recordings of isolated CM indicated that T3 increased rates of Ca2+ release and re-uptake, resulting in increased velocities of sarcomere shortening and re-lengthening. TT periodicity was significantly decreased, with reduced transverse tubules but increased longitudinal tubules in TH-deficient CMs and LV tissue, and these structures were normalized by T3 treatment. Analysis of STORM data of PTU myocytes showed decreased RyR2 cluster numbers and RyR localizations within each cluster without significant changes in Cav1.2 localizations within RyR clusters. T3 treatment normalized RyR2 cluster size and number. qPCR and RNAseq analyses of LV and cultured CM showed that Jph2 expression was T3-responsive, and its increase with treatment may explain improved TT organization and RyR-LTCC coupling.]]>
Journal of Molecular and Cellular Cardiology, Volume 160, November 2021, Pages 1-14]]>
https://repository.nyitlibrary.org/files/original/799371860b31fbd59d1b16acddf4de08.pdf]]>
https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
Gilani, N., Wang, K., Muncan, A., Peter, J., An, S., Bhatti, S., Pandya, K., Zhang, Y., Tang, Y.-D., Gerdes, A. M., Stout, R. F., & Ojamaa, K. (2021). Triiodothyronine maintains cardiac transverse-tubule structure and function. In Journal of Molecular and Cellular Cardiology (Vol. 160, pp. 1–14). Elsevier BV. https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
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Ca(2+)
Calcium Channels, L-Type metabolism
Calcium Signaling drug effects
Calcium metabolism
Cells, Cultured
Disease Models, Animal
Female
Gene Expression
Heart Ventricles drug effects metabolism
Hypothyroidism blood chemically induced drug therapy
Index Medicus
Junctophilin-2
Membrane Proteins genetics metabolism
Myocytes, Cardiac metabolism
Rats
Ryanodine Receptor Calcium Release Channel metabolism
Ryanodine receptors
STORM
SY7Q814VUP
Sarcolemma metabolism
Sarcomeres metabolism
Thyroid hormone
Transverse-tubules
Treatment Outcome
Triiodothyronine administration & dosage blood
Ventricular Function drug effects]]>
Thyroid hormone (TH) deficiency was induced in adult female rats by propyl-thiouracil (PTU; 0.025%) treatment for 8 weeks. Rats were then randomized to continued PTU or triiodo-L-thyronine (T3; 10 μg/kg/d) treatment for 2 weeks (PTU + T3). After in vivo echocardiographic and hemodynamic recordings, cardiomyocytes (CM) were isolated to record Ca 2+ transients and contractility. TT organization was assessed by confocal microscopy, and STORM images were captured to measure ryanodine receptor (RyR2) cluster number and size, and L-type Ca 2+ channel (LTCC, Ca v 1.2) co-localization. Expressed genes including two integral TT proteins, junctophilin-2 (Jph-2) and bridging integrator-1 (BIN1), were analyzed in left ventricular (LV) tissues and cultured CM using qPCR and RNA sequencing.
The T3 dosage used normalized serum T3, and reversed adverse effects of TH deficiency on in vivo measures of cardiac function. Recordings of isolated CM indicated that T3 increased rates of Ca 2+ release and re-uptake, resulting in increased velocities of sarcomere shortening and re-lengthening. TT periodicity was significantly decreased, with reduced transverse tubules but increased longitudinal tubules in TH-deficient CMs and LV tissue, and these structures were normalized by T3 treatment. Analysis of STORM data of PTU myocytes showed decreased RyR2 cluster numbers and RyR localizations within each cluster without significant changes in Ca v 1.2 localizations within RyR clusters. T3 treatment normalized RyR2 cluster size and number. qPCR and RNAseq analyses of LV and cultured CM showed that Jph2 expression was T3-responsive, and its increase with treatment may explain improved TT organization and RyR-LTCC coupling.]]>
Journal of Molecular and Cellular Cardiology, Volume 160, November 2021, pages 1-14]]>
https://repository.nyitlibrary.org/files/original/09a4bcbf409ddd13afe0348f2f4a2b4c.pdf]]>
https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
Gilani, N., Wang, K., Muncan, A., Peter, J., An, S., Bhatti, S., Pandya, K., Zhang, Y., Tang, Y.-D., Gerdes, A. M., Stout, R. F., & Ojamaa, K. (2021). Triiodothyronine maintains cardiac transverse-tubule structure and function. In Journal of Molecular and Cellular Cardiology (Vol. 160, pp. 1–14). Elsevier BV. https://doi.org/10.1016/j.yjmcc.2021.06.010]]>
Diabetics--Nutrition]]>
https://repository.nyitlibrary.org/files/original/b215616a2ee651afc6f2217a499fa469.pdf]]>
Energy policy.
Energy conservation.
]]>
https://repository.nyitlibrary.org/files/original/03491b8acf86574352ee2e6805155e98.pdf]]>
Interpersonal conflict.]]>
https://repository.nyitlibrary.org/files/original/4f7abfbacc5d1c3c6428bb5ead0726fb.pdf]]>
Investments, American.]]>
https://repository.nyitlibrary.org/files/original/1eaac6e90139121068ad93f2e7b16281.pdf]]>
https://repository.nyitlibrary.org/files/original/1e0d279279a1ffbe49876ad40ae8ec5a.pdf]]>
https://repository.nyitlibrary.org/files/original/3a184001aab5f00d35812b1d5d81910c.pdf]]>
https://repository.nyitlibrary.org/files/original/46c2ee21df6b689a230bc064514de9a5.pdf]]>
Airplanes--Marketing.
Airplanes, Military--Marketing.]]>
https://repository.nyitlibrary.org/files/original/50ce2b10bc4f53281f200f3c2fe0272e.pdf]]>
Financial statements.]]>
https://repository.nyitlibrary.org/files/original/eb9aacad3cd17ab525c1ab662fb54da6.pdf]]>
Ocean Waves]]>
https://repository.nyitlibrary.org/files/original/c7372a41a193edbf615182e2597d083a.pdf]]>
https://repository.nyitlibrary.org/files/original/141061bbb6dc73ad6e0853b6fa17d4d7.pdf]]>
https://repository.nyitlibrary.org/files/original/d1dc9e15f13f747a62541070bd8c6f29.pdf]]>
Public utilities --Costs.]]>
https://repository.nyitlibrary.org/files/original/26ef200a11e7edcc9f429edf27bd7144.pdf]]>
Water treatment plants.
Water--Purification.]]>
https://repository.nyitlibrary.org/files/original/d124a3d98fa989cf9ed1834993934ab8.pdf]]>
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Cetacean vertebrae
Failed predation
Harmful algal bloom
Miocene
Otodus megalodon
Seizure
Shear-compression fracture
Acid
Algal bloom
Cetacean
Compression
Fracture
Miocene
Pathology
Predation
Shark
Shear
Tooth
Toxicity]]>
A single megatoothed shark tooth from Otodus megalodon was found with the vertebra. It is not known if the tooth came to be there serendipitously, or if it was associated because it was lost as a result of the possible originating failed predation event, or during a final successful predation or subsequent scavenging event.
The fractures are severe and unlikely to have had an endogenous origin like convulsions, seizures, or spasms. Seizures can cause vertebral fractures in humans, including elderly adults with poor bone health as well as physically fit younger individuals. Seizures causing injuries of this magnitude have not been observed in cetaceans, though domoic acid toxicity from harmful algal blooms are known to cause seizures in cetaceans, and are implicated in the deaths of neonatal skim-feeding mysticetes. It is unlikely, but possible, that a large mysticete would be affected by domoic acid toxicity to the point of a spinal fracture-causing seizure. Similarly, protozoal infections are known to cause seizures in cetaceans, though physical diagnosis of this is impossible in a fossil. Partly healed bone fractures of the face from possible collisions with the seafloor have been reported from fossil mysticetes of shallower regions of this fauna, but a spinal fracture this far back in the spinal column seems unlikely to be the result of a seafloor collision. Even though the cause of the vertebral hyperflexion and resulting trauma is unknown, a plausible cause was a crushing ambush delivered by a macropredatory shark or macroraptorial physeteroid. In spite of extant cetaceans being subjected to anthropogenically-induced trauma, which include vessel-strike blunt force injuries of many different kinds, shear-compression fractures and periosteal reactions like the ones detailed here have not yet been reported in extant cetaceans. Therefore, we consider the fracture as likely due to an impact from a predator, such as Otodus megalodon, or possibly from seizures due to a harmful algal bloom and resulting domoic acid toxicity. In either scenario, the cetacean survived.]]>
Palaeontologia Electronica, Article 25.3.a28, September 2022]]>
https://repository.nyitlibrary.org/files/original/625ed29d2da781f30e49559144d0beca.pdf]]>
https://doi.org/10.26879/1171]]>