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                <text>Bidirectional ventricular tachycardia&#13;
Caffeine&#13;
Dantrolene&#13;
Dobutamine&#13;
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                <text>&lt;h3&gt;Copyright&lt;/h3&gt;
&lt;div class="copyright"&gt;© 2020 Heart Rhythm Society. Published by Elsevier Inc.&lt;/div&gt;
&lt;h3&gt;User license&lt;/h3&gt;
&lt;a href="http://creativecommons.org/licenses/by-nc-nd/4.0/" class="userLicLnk"&gt;Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0)&lt;/a&gt;&lt;span&gt; &lt;/span&gt;|&lt;span&gt; &lt;/span&gt;
&lt;div class="dropBlock reference-citations"&gt;&lt;a href="https://www.heartrhythmopen.com/article/S2666-5018(20)30114-8/fulltext#" class="reference-citations__ctrl"&gt;How you can reuse&lt;/a&gt;&lt;span&gt; &lt;/span&gt;&lt;/div&gt;</text>
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                <text>&lt;div&gt;
&lt;h4 class="u-h4 u-margin-m-top u-margin-xs-bottom"&gt;Background&lt;/h4&gt;
&lt;p&gt;Bidirectional&lt;span&gt; &lt;/span&gt;&lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/ventricular-tachycardia" title="Learn more about ventricular tachycardia from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;ventricular tachycardia&lt;/a&gt;&lt;span&gt; &lt;/span&gt;(BD-VT) is an intriguing arrhythmia, characterized by a beat-to-beat alternation of the QRS polarity on electrocardiogram. Currently there is no simple BD-VT animal model.&lt;/p&gt;
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&lt;div&gt;
&lt;h4 class="u-h4 u-margin-m-top u-margin-xs-bottom"&gt;Objective&lt;/h4&gt;
&lt;p&gt;&lt;span&gt;We report a simple animal model of BD-VT induced by caffeine and &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/dobutamine" title="Learn more about dobutamine from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;dobutamine&lt;/a&gt; (C+D) challenge in normal rats in which the arrhythmia can be attenuated by &lt;/span&gt;&lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/dantrolene" title="Learn more about dantrolene from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;dantrolene&lt;/a&gt;&lt;span&gt; (a &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/ryanodine-receptor" title="Learn more about ryanodine receptor from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;ryanodine receptor&lt;/a&gt; stabilizer) &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/therapeutic-procedure" title="Learn more about treatment from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;treatment&lt;/a&gt;, but not by the pacemaker &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/channel-blocker" title="Learn more about channel blocker from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;channel blocker&lt;/a&gt; &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/ivabradine" title="Learn more about ivabradine from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;ivabradine&lt;/a&gt; treatment.&lt;/span&gt;&lt;/p&gt;
&lt;/div&gt;
&lt;div&gt;
&lt;h4 class="u-h4 u-margin-m-top u-margin-xs-bottom"&gt;Methods&lt;/h4&gt;
&lt;p&gt;Adult (4–5 months old) Sprague-Dawley rats (both sexes) were randomized into C+D (n = 8, received caffeine 120 mg/kg intraperitoneally [IP] and dobutamine 60 μg/kg IP, sequentially) and control (n = 8) groups. In addition, a group of 7 rats were pretreated with dantrolene (10 mg/kg, IP) 30 minutes before the C+D challenge and another group of 8 rats were pretreated with ivabradine (5 mg/kg, IP) 30 minutes before the C+D challenge.&lt;/p&gt;
&lt;/div&gt;
&lt;div&gt;
&lt;h4 class="u-h4 u-margin-m-top u-margin-xs-bottom"&gt;Results&lt;/h4&gt;
&lt;p&gt;&lt;span&gt;C+D challenge induced spontaneous &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/premature-ventricular-contraction" title="Learn more about premature ventricular contractions from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;premature ventricular contractions&lt;/a&gt; (PVCs) in 7 of 8 rats and BD-VT (lasted 4.3 ± 2.9 minutes, terminated spontaneously) in 6 of 8 (75%) rats. No &lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/heart-ventricle-arrhythmia" title="Learn more about ventricular arrhythmia from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;ventricular arrhythmia&lt;/a&gt; was induced in the control group (&lt;/span&gt;&lt;em&gt;P&lt;/em&gt;&lt;span&gt; &lt;/span&gt;&amp;lt; .05 vs C+D group). Dantrolene treatment significantly decreased BD-VT (1 of 7 rats in the Dantrolene+C+D group vs 6 of 8 rats in C+D group,&lt;span&gt; &lt;/span&gt;&lt;em&gt;P&lt;/em&gt;&lt;span&gt; &lt;/span&gt;&amp;lt; .05). Ivabradine treatment did not affect C+D-induced BD-VT (7 of 8 rats in the Ivabradine+C+D group vs 6 of 8 in the C+D group,&lt;span&gt; &lt;/span&gt;&lt;em&gt;P&lt;/em&gt;&lt;span&gt; &lt;/span&gt;&amp;gt; .05).&lt;/p&gt;
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&lt;div&gt;
&lt;h4 class="u-h4 u-margin-m-top u-margin-xs-bottom"&gt;Conclusion&lt;/h4&gt;
&lt;p&gt;Caffeine and dobutamine challenge induces BD-VT in a majority of normal rats. Stabilizing&lt;span&gt; &lt;/span&gt;&lt;a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/ryanodine-receptor-2" title="Learn more about cardiac ryanodine receptors from ScienceDirect's AI-generated Topic Pages" class="topic-link"&gt;cardiac ryanodine receptors&lt;/a&gt;&lt;span&gt; &lt;/span&gt;with dantrolene treatment can significantly decrease the occurrence of BD-VT, but pacemaker channel blocker ivabradine treatment does not have effect in this animal model.&lt;/p&gt;
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